제품 상세
병원성 균의 생존력을 연장시키기 위해 숯(charcoal)이 포함된 수송배지
Typical Formula* | gm/litre |
Charcoal pharmaceutical | 10.0 |
Sodium chloride | 3.0 |
Sodium hydrogen phosphate | 1.15 |
Potassium dihydrogen phosphate | 0.2 |
Potassium chloride | 0.2 |
Sodium thioglycollate | 1.0 |
Calcium chloride | 0.1 |
Magnesium chloride | 0.1 |
Agar | 4.0 |
pH 7.2 + 0.2 @ 25°C |
* Adjusted as required to meet performance standards
Directions
Suspend 20 g in 1 litre of distilled water. Bring to the boil to dissolve the agar completely. Distribute into small, screwcap bottles, stirring meanwhile to keep the charcoal evenly suspended. Screw down the caps firmly on the completely filled bottles. Sterilise by autoclaving at 121°C for 15 minutes. Invert the bottles whilst cooling to distribute the charcoal uniformly. Store in a cool place.
Appearance
Dehydrated medium: Black coloured, free-flowing powder
Prepared medium: Straw coloured semi-solid gel
Quality control
Positive controls: | Expected result at 35°C |
Staphylococcus aureus ATCC® 25923 * | Good growth |
Escherichia coli ATCC® 25922 * | Good growth |
Negative control: |
|
Uninoculated medium | No change |
* This organism is available as a Culti-Loop®
Precautions
It is important that the charcoal is properly suspended in the medium, invert the bottles when the bottles are cool but the agar still liquid.
During preparation of the medium, avoid prolonged heating in open flasks because thioglycollate is volatile.
Old medium should be freshly steamed and the charcoal resuspended before use.
Keep medium cool during transport but do not freeze.